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Abstract
The hawkmoth genus Ambulyx is one of the complex genera in the family Sphingidae (Subfamily: Smerinthinae). Ambulyx siamensis and Ambulyx pryeriare found in many parts of Thailand. Both species exhibit very similar morphological characteristics, making identification challenging, especially for non-experts. Geometric morphometrics is an inexpensive tool that has been developed and widely applied for size and shape analyses in various fields including taxonomy and evolutionary studies. This study aimed to examine the possibility of using a geometric morphometric approach to discriminate two species of Ambulyx and distinguish the geographical locality of the specimens. Atotal of 50 pinned specimens, including 32 specimens of A. siamensis and 18 specimens of A. pryeri, from the Natural History Museum, National Science Museum, Thailand were imaged and digitized. Fourteen landmarks on the right forewing were chosen and used as the primary morphometric dataset to represent wing shape variations. Acanonical variate analysis was performed to examine variation between species and localities. The results indicated a high accuracy of species identification between A. siamensis (83.33%) and A. pryeri (71.88%).Interestingly, specimens localities could potentially be specified using geometric morphometrics, with total an accuracy of 70.97% and 87.50% for A. siamensis and A. pryeri, respectively. These findings suggest that geometric morphometrics is an effective approach for determining species and localities of A. siamensis and A. pryeri, potentially supporting future studies on hawkmoths.
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References
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