Isopod crustaceans are common benthos, widely distributed in terrestrials and aquatic habitats. This study aims to explore the species diversity of isopods in Amphawa Estuary, Samut Songkhram Province, during March – June 2022. The isopods were sampled by Ekman grab and seven artificial settlement pads, i.e., fine filter, coarse filter, flute board, coconut husk, oyster shell, doormat, and brush. A total of 4 species from 3 families were found. The dominant species was Ischyromene sp. The result revealed that Bangchang Creek contained the highest diversity, followed by Soi Mangkorn Tong, Ampawan Wittayalai School, and Darunanukrao School.